• Semen specimen produced by masturbation only, collected into a sterile, non-toxic, pre-weighed, wide-mouthed clear plastic container provided by the laboratory with a hand written label. Note: Any lost fraction of sample can be recorded on the patient’s pre-analysis questionnaire (questionnaire provided by the laboratory).
• Non-toxic condoms may only be used for sample collection in exceptional circumstances, such as when it is evident that the patient has an inability to produce a sample by masturbation and only after the GP has discussed these circumstances with the laboratory prior to the patient booking an appointment.
• The patient needs to have abstained from intercourse/ejaculation for 48 hours prior to sample collection, but needs to have had intercourse or ejaculated at least once, 5 days prior to the 48-hour abstinence period, for an abstinence period to meet the 7-2 days’ requirement.
• Patients will be recommended to produce their samples on-site in the available onsite production room facility available at both NNUH and QEHKL in order to ensure their sample is tested within 60 minutes. Samples produced at home must be delivered well within 60 minutes of production, ideally at 30 minutes’ post sample production so the start time of analysis can commence within 60 minutes in accordance with the WHO Guidelines.
• There is no minimum sample volume required – samples of low volume may be of clinical significance. 
• Containers are supplied to the patient by the laboratory and need to be labelled with the patient’s full name, date of birth and HRN/NHS number by hand and not using any additional pre-labelled stickers found on the ICE form (ICE form stickers are for internal laboratory use only) as this affects the final sample volume – The sample container labelling will be carried out by the laboratory if the patient is producing their sample on site.
• A pre-analysis questionnaire needs to be completed and signed by the patient.

Rejection of samples that do not meet the above requirements: Basic Semen Analysis & Vasectomy Reversal Tests

• The entire sample has leaked in transit (Basic Semen Analysis). Partial loss of sample is acceptable for Basic Semen Analysis with a record of the lost fraction on the patient-pre analysis questionnaire.
• Samples that are dropped off at the laboratory reception when the patient has not attended his sample delivery appointment and there is no patient pre-analysis form filled out.
• Unlabelled/incorrectly labelled samples (for example those that are dropped off at the laboratory reception when the patient has not attended his sample delivery appointment). 
• Samples that arrive at the laboratory when the patient does not have an appointment and the workload in the laboratory does not allow time for the sample to be tested.
• Samples that arrive at the laboratory through the EPA community GP blood sample transport system.
• The patient does not have a valid request from a clinician for the test.
• If a sample has been brought to the sample delivery appointment by a representative and the pre-analysis questionnaire has not been signed by the patient.
• Samples that are not produced on the same day as their scheduled appointment.

Post Vasectomy Tests

• Must be at least 12 weeks’ post-procedure and after a minimum of 20 ejaculations. NOTE: If samples are received earlier than 12 weeks post procedure, the test will need to be repeated.
• A complete ejaculate of semen produced my masturbation only, collected into a pre-weighed non-toxic container provided by the laboratory.

NOTE: Incomplete samples & samples that have leaked in transit cannot be tested and will be rejected. 

NOTE: Samples produced in any other container than that provided by the laboratory will be rejected.  

• The patient must have abstained from intercourse/ejaculation for 48 hours prior to sample collection, but needs to have had intercourse or ejaculated at least once, 5 days prior to the 48-hour abstinence period, for an abstinence period to meet the 7-2 days’ requirement

NOTE: If the patient has not adhered to the abstinence period the sample will be rejected, and they will be required to repeat the test.

• Patients will be recommended to produce their samples on-site in the available onsite production room facility available at both NNUH and QEHKL in order to ensure their sample is tested within 60 minutes. At home sample production is not recommended for post vasectomy semen analysis tests.

NOTE: If immotile cells are present in the sample and the analysis has not taken place within 60 minutes the test will need to be repeated.

• Containers are supplied to patient by the laboratory and need to be labelled with the patient’s full name, date of birth and HRN/NHS number by hand and not using any additional pre-labelled stickers found on the ICE form (ICE form stickers are for internal laboratory use only) as this affects the final sample volume – The sample container labelling will be carried out by the laboratory if the patient is producing their sample on site.
• A pre-analysis questionnaire needs to be completed and signed by the patient.

Rejection of samples that do not meet the above requirements: Post Vasectomy Tests

• Incomplete samples & samples that have leaked in transit/sample incubator in onsite patient production room.
• Samples received in a container other than that provided by the laboratory.
• Samples produced in a non-toxic condom.
• Samples older than 4 hours old at the time of sample delivery.

Note: If any Immotile cells are seen in the sample and the sample is tested >60 mins post collection the patient will need to repeat that test as those results will be invalid.

• Sample received sooner than 12 weeks post vasectomy procedure.
• Samples where the patient has not adhered to the recommended abstinence period.
• Samples that are dropped off at the laboratory reception when the patient has not attended his sample delivery appointment and there is no patient pre-analysis form filled out.
• Unlabelled/incorrectly labelled samples (for example those that are dropped off at the laboratory reception when the patient has not attended his sample delivery appointment). 
• Samples that arrive at the laboratory when the patient does not have an appointment and the workload in the laboratory does not allow time for the sample to be tested.
• Samples that arrive at the laboratory through the EPA GP blood sample transport system.
• The patient does not have a valid request from a clinician for the test.
• If a sample has been brought to the sample delivery appointment by a representative and the pre-analysis questionnaire has not been signed by the patient.

Medical officers responsible for the care of patients have a duty of care towards other members of staff – therefore all samples from patients who are known to have or strongly suspected of having the conditions noted below must be identified. 

• Creutzfeldt-Jakob disease (CJD)
• Viral haemorrhagic fever (VHF) of any type
• Microorganisms, (biological agents) in Hazard Group 3 or 4.
• Pyrexia of unknown origin (PUO) recently returned from Africa.

Medical staff should ensure that appropriate information, including relevant travel history, is provided in order to alert laboratory staff of potential dangers. Clinical details supplied on sample request forms must contain clear information regarding the nature of the test being requested and sufficient detail to inform laboratory staff upon the safety precautions they need to take in order to process the sample without risk of infection.

If, during patient intervention, further information becomes available that has implications for the safety of laboratory staff this must be communicated immediately to the laboratory so that appropriate steps regarding containment can be taken.

Norfolk and Norwich University Hospital

Mon–Fri 09:00–17:00

Tel 01603 289847 (Ext: 5847)

Email: [email protected]

Queen Elizabeth Hospital

Mon–Fri 08:00–17:00

Tel (via switchboard 01553 613613) Ext: 2596

Email: [email protected]

• Basic Semen Analysis appointments: are usually booked a minimum of 10 days in advance to allow sufficient time for patients to read the information leaflets and adhere to the sample collection instructions, and if the sample is being produced at home, this provides enough time for posting the sample pack directly to the patient’s home address.
• Post Vasectomy Semen Analysis appointments should be no sooner than 12 weeks’ post-procedure and after a minimum of 20 ejaculations (Note: the laboratory takes no responsibility for the monitoring or recording of this recommendation that analysis takes place after ‘a minimum of 20 ejaculations’ as stated by the 2016 Post Vasectomy Semen Analysis Guidelines). It is also the responsibility of the patient to ensure their appointment takes place after 12 weeks post vasectomy procedure. If their analysis has taken place sooner, they will be asked to repeat the test.

NOTE: Please advise patients to book their 12-week appointment in advance immediately after their procedure due to the long appointment waiting list.

Appointments are only available to book after a valid request has been electronically generated in Web ICE or manually completed by a clinician. Appointments are to be made via the online DrDoctor appointment booking service. Please use the link below.

Andrology streaming form – Formstack

Or Scan the QR Code:

The website link is provided on the request form (if requested electronically on Web ICE) or the patient information leaflet (if requested manually). It can also be found on the NNUH and QEHKL Trust websites under ‘Andrology’. Patients can search ‘NNUH Andrology’ or ‘QEHKL Andrology’ in Google to find the link to these pages.

Patients will receive a SMS text message or email confirmation of their appointment and a link to the patient information leaflets once they have booked.  

 *Please ensure the Patient reads the information leaflet carefully 7 days prior to the appointment* There are specific sample requirements the patient needs to be aware of prior to their appointment. Patients will also need to bring the request form the GP/Clinician has provided to the appointment.

 If the patient is unable to use the online system, they can ask someone to book an appointment on their behalf; or please inform them to telephone the laboratory on 01603 646524. The patient will need to confirm their full name, DOB, HRN/NHS number and

address when booking their appointment via the telephone and the location of the laboratory that they require their sample to be tested at; appointments are made centrally by the pathology office at the NNUH for both EPA laboratory locations.

NOTE: If a patient arrives more than 15 minutes late to their appointment the laboratory may not be able to continue to accommodate the appointment, and they may be turned away and asked to re-book an alternative appointment via the online appointment booking service.

We recommend patients produce their sample on site in our sample production room facility that is located close to the laboratory in order to ensure the sample is tested within 60 minutes of production, as this increases the reliability of the results that the laboratory issues to the clinician.

When the patient arrives at the Pathology reception, they will be greeted by an Andrology staff member who will escort them to the private on site production room and explain everything the patient needs to be aware of. The laboratory will provide the patient with a labelled container and a pre-analysis questionnaire to complete. 

NOTE: Due to the strict requirements for Post Vasectomy samples – this option is not recommended for Post Vasectomy Semen Analysis Tests.

If a patient lives within a 30-minute radius of the NNUH or the QEHKL there is an option for their sample for Basic Semen Analysis to be produced at home and delivered to the laboratory, however the sample delivery needs to take place ideally at 30-45 minutes’ post sample collection to ensure the sample is tested within the recommend time frame of <60 minutes. 

The laboratory will send a sample pack directly to the patient’s home address after the appointment has been made if this option is selected. This pack will contain the following:

 Pre-weighed non-toxic sample container

• Pre-analysis questionnaire
• Information leaflet containing clear instructions
• ‘What We Test Your Sample for’ leaflet

1. Please ensure the sample is kept as close to body temperature (37°C) as possible by keeping the sample close to the body in a trouser/coat pocket during transport to the laboratory.
2. Please ensure the pre-analysis questionnaire has been correctly completed and signed by the patient.
3. Please ensure the request form is brought to the sample delivery appointment.
4. Samples may be delivered by a partner or representative only if the patient has labelled the sample container with three points of identification and correctly completed and signed the pre-analysis questionnaire. A patient’s partner/representative must bring the patient’s request form to the sample delivery appointment, and must adhere to the sample delivery requirements stated above and in the patient information leaflet.

Note: Samples delivered by a representative where the patient has not signed the patient’s pre-analysis questionnaire will be rejected.